Core C: Electrophysiology Core

Project Summary/Abstract The Electrophysiology Core (Core C) will provide services for in vitro electrophysiological studies using novel tools such as optogenetics and in vitro GCaMP6 calcium imaging. We will prepare brain slices, conduct patch- clamp recordings, channelrhodopsin2 (ChR2)-assisted circuit mapping (CRACM), and Ca2+ imaging studies in brain slices. The Core will also perform histology of the recorded slices and analyze the associated data. Project 1 (Saper), Project 2 (Scammell), Project 3 (De Luca), and Project 4 (Vanderhost and Dymecki) will use this Core facility. These projects are not equipped to conduct in vitro electrophysiology recordings, and there are no institutional resources that can provide this service. These projects will benefit from the expertise and shared resources provided by the Core.