Molecular Immunology Core

CORE SUMMARY / ABSTRACT The MIC will provide a centralized hub for advanced cellular and systems immunological analysis of all biologic samples derived from Projects 1-2. In addition, the MIC will coordinate and streamline all common assays and procedures to ensure that both Projects utilize uniform Standard Operating Procedures (SOPs) and best practices. The MIC is comprised of five interacting sub-Cores: (1) The MHC Immunogenetics and Typing Core; (2) the Flow Cytometry and CyTOF Core; (3) The Virology and Functional Protective Immunity Core; (4) The Molecular Immunology and Transcriptomics Core; and (5) The Computational Analysis Core. Each Core addresses an essential requirement for the optimal conduct of the complex experiments that are proposed in Projects 1-2 and promotes collaboration between investigators. As such, the MIC will significantly increase the efficiency and the rigor with which our group is able to execute the lung transplant tolerance experiments proposed in this U19.