Non-invasive Therapy using Lamina Propria Extract for Vocal Fold Healing

PROJECT SUMMARY To address the public health issue of voice disorders affecting approximately 20 million Americans annually due to vocal fold (VF) scarring and laryngotracheal injuries, this project focuses on a non-invasive, innovative treatment strategy. Utilizing a specially derived Vocal Fold Lamina Propria extract (VFLPx), which has demonstrated promising results in reducing fibrotic gene expression, the proposed project aims to fine-tune the dosage and application methods of VFLPx. The research is structured around three principal objectives: (1) To conduct a detailed investigation into the anti-fibrotic and anti-microbial properties of VFLPx through advanced proteomic analysis and targeted assays, identifying the key molecular constituents responsible for its therapeutic effects. (2) To assess the efficacy of VFLPx in promoting wound healing within a rabbit model of VF injury, exploring its use both as a singular treatment option and in conjunction with aerosolized forms, aiming to minimize the reliance on invasive surgical interventions. This will include evaluating the benefits of both direct injection and aerosolized delivery to determine the most effective approach for tissue repair. (3) To rigorously evaluate the safety of nebulized VFLPx in a rat model, particularly its impact on lung function and the respiratory system, to ensure its suitability for long-term use in human patients. The project’s ultimate goal is to establish a solid foundation for developing a less invasive, more patient-friendly approach to treating VF and laryngeal injuries, potentially revolutionizing the management of such conditions and significantly improving patient outcomes.