ANNOUNCEMENT

NOBEL PRIZE 2026

This year's laureates, their fields, and the discoveries that earned them the prize.Live nowSee the winners
Moonbase
← Back to Awards
NIHNATIONAL CANCER INSTITUTE

CRISPR Tiling

Active
Institution
ST. JUDE CHILDREN'S RESEARCH HOSPITAL, TN
Principal investigator
Chun-Wei David Chen
Funding
$518K
Duration
2025–2030
Open portfolio

Moonbase score

Still being scored

Abstract

PROJECT SUMMARY- CORE 2 Fusion oncoproteins (FOs) enclose folded domains and intrinsically disordered regions (IDRs) rearranged from fusion partner genes, contributing to condensate formation and oncogenic transformation. Understanding of functional elements within FOs will elaborate on how FOs promote aberrant gene expression and other types of chromatin deregulation in cellular cancer model systems. The objective of Core 2 is to serve as a genetic platform for evaluating the FOs under study in Projects 1, 2, and 3. Based on the recently reported “CRISPR/Cas9 screening of protein domains”, Core 2 has optimized a high-density CRISPR gene scan pipeline termed “CRISPR tiling.” This platform uses libraries of sgRNAs to exhaustively and individually disrupt all 2-3 amino acid long regions within proteins of interest, followed by testing for the contribution of the disrupted region to protein function. The proposed CRISPR tiling strategy is innovative because it will provide a comprehensive and non- biased evaluation of FO coding regions, thereby pinpointing critical elements within folded domains and IDRs for FO-driven malignancies. The impact of Core 2 will be significant as it will assist with Projects 1, 2, and 3 in identifying novel mechanisms and therapeutic opportunities within the investigated FOs.

LOADING MOONBASE SCORE

NATIONAL CANCER INSTITUTEP01Special Emphasis Panel[ZCA1 RPRB-T (J1)]domainscrisprregionsoncoproteinsfusionfoscancer modelfolded

Questions for ST. JUDE CHILDREN'S RESEARCH HOSPITAL

Answered by the team behind this research.

No questions yet. Sign in to ask the first one

Related grants

Same researchers, same institution, same topic, scored first.