CSHL Single Cell Analysis Course (2023-2027)

ABSTRACT CSHL Laboratory Course in Single Cell Analysis The proposed Cold Spring Harbor Laboratory (CSHL) course Single Cell Analysis to be held annually in July 2023– 2027, is part of the CSHL’s postgraduate training program in cell and molecular biology. This is a short, intensive 2 week hands-on course which prepares students to enter directly into research that makes use of advanced and/or specialized molecular techniques in contemporary aspects of singe cell-based research. General themes to be covered during the course include quantitative single cell analysis by RNAseq, genomic DNA analysis, proteomics, and metabolomics. Specific experimental techniques taught in the course include: 1) Droplet- and microwell based single cell isolation; 2) Single cell RNA seq library preparation; 3) Single molecule FISH; 4) Single cell peptidomics; 5) Single cell western blot; 6) Photoactivatable single cell probes; 7) Single cell mass spectrometry; 8) Soft X-ray tomography; 9) Introductory single cell sequencing analysis. Experimental techniques are taught in the context of the broader conceptual advances that they may provide. The course invites lecturers who have made significant contributions in their fields to give up-to-the-minute reports on current research. The trainees are chosen by the course faculty from larger pools of applicants and range from graduate students to senior investigators and research physicians. Because of the short duration of this course, senior, as well as junior individuals can attend and receive a short, intense period of training in an environment remote from other demands on their time and attention. The course also provides an unusual opportunity for established scientists to apply these advanced techniques to their own research interests, or to retrain in an important specialty within neuroscience with which they may not be familiar. Methods and concepts taught in the course are disseminated to the wider research community through the publication of laboratory manuals and online resources.