DNA Transistors for Cellular Programming

PROJECT SUMMARY/ABSTRACT The "DNA Transistors for Cellular Programming" project aims to revolutionize synthetic biology by developing tools that enable precise, modular, and multiplexed control over cellular functions. Utilizing DNA-binding domains from nuclease technologies, such as TALENs and zinc finger nucleases, our research focuses on creating DNA-based transistors capable of operating both extracellularly and intracellularly. This dual functionality permits unprecedented manipulation of cellular activities through specific DNA sequences, whether inherently present or introduced by researchers. Our project is structured around two primary goals: the development of Extracellular DNA Transistors for Signal Interpretation and Intracellular DNA Transistors to Regulate Cell Function. The first goal aims to engineer synthetic membrane receptors that respond to specific DNA sequences, allowing precise control of cellular responses to both introduced and naturally occurring cell-free DNA. The second goal concentrates on internally manipulating cellular processes by using targeted DNA sequences to regulate the activity of enzymes and proteins, offering a highly versatile and programmable approach to cellular control. This groundbreaking approach not only enhances our understanding of cellular dynamics but also paves the way for innovative therapeutic interventions. By offering a method to program cells with high specificity and minimal off-target effects, our project holds the potential for significant advances in disease treatment and tissue engineering. If successful, this research could establish new benchmarks in the control and programming of cellular functions, echoing the transformative impact of electronic transistors in computing.