ADRDL Diagnostic testing support for the FDA CVM VET-LIRN program

4/12/23 Project Summary The South Dakota Animal Disease Research and Diagnos�c Laboratory (SD ADRDL) will serve as a Whole Genome Sequencing (WGS) core laboratory for the Veterinary Laboratory Inves�ga�on and Response Network (Vet-LIRN). The main aim of this project is to perform WGS on isolates obtained from working with 5 partner source labs: North Carolina State University Center for Veterinary Medicine, Michigan State University Veterinary Diagnos�c Laboratory, Minnesota Veterinary Diagnos�c Laboratory, Iowa State University Veterinary Diagnos�c Laboratory, and Oklahoma Animal Disease Diagnos�c Laboratory. These partners will culture Salmonella enterica, Escherichia coli, Staphylococcus pseudintermedius, and other relevant organisms from veterinary matrices. Once sent to the SD ADRDL, iden�ty will be verified via MALDI-TOF before extrac�ng genomic DNA and performing WGS. Sequence will be uploaded to NCBI bioprojects designated by Vet-LIRN Program Office. Vet-LIRN has been tasked with developing AST and WGS assays that will allow for increased understanding of an�microbial resistance within veterinary samples. This knowledge will be used to develop evidence-based interven�ons that have demonstrated reduc�ons in resistance. 5U18FD006558 Page 1 of 1