Advanced Microscopy Shared Resource

PROJECT SUMMARY: ADVANCED MICROSCOPY SHARED RESOURCE The UNMC Advanced Microscopy Core Facility (UAMCF) at the University of Nebraska Medical Center is dedicated to providing top-notch microscopy and imaging services for competitive fees. The UAMCF was launched in August 2014 to replace the previous campus microscopy core facility (Confocal Laser Scanning Microscopy Facility: CLSMF) with the goal of acquiring super-resolution microscopy capabilities on campus. UAMCF currently provides users with access to a dual-platform super-resolution microscope with capabilities for super-resolution Structured Illumination Microscopy (sr-SIM) and single molecule Photoactivatible Localization Microscopy/direct Stochastic Optical Reconstruction Microscopy. Sr-SIM capabilities include up to 100 nm resolution in the x/y axis, and ~300 nm axial resolution. PALM/dSTORM capabilities include single molecule imaging and precision of ~5 nm coupled with ~10 nm Nyquiste Resolution. In addition, two recent- model Zeiss confocal microscopes, fully equipped for live image analysis and complete with adjacent workstations are also available. The Zeiss LSM 800 confocal microscope also has an Airyscan unit for enhanced resolution that approaches that of sr-SIM imaging. The facility provides outstanding service including consultations by active researchers with expertise who have published studies using advanced microscopy techniques. In addition, a Zeiss Cell Discoverer 7 microscope is available for a variety of automated studies. Finally, the facility also boasts a complete Imaris workstation for advanced 3D imaging processing and quantification. Overall, the UAMCF provides outstanding support for Cancer Center members and other researchers on and off campus.