Microbial Genomics and Analytical Laboratory (MGAL) Core

PROJECT SUMMARY – Microbial Genomics and Analytical Laboratory (MGAL) The Microbial Genomics and Analytical Laboratory (MGAL) Core of the Integrative Center for Environmental Microbiomes and Human Health provides a centralized facility and technical expertise at the University of Hawai‘i at Mānoa (UHM) to support the study of the interface between the microbial environment and human health. The characterization of microbiota community composition by high throughput sequencing and metabolite analysis are standard techniques used in the field of microbiome research. The MGAL Core enables access to these key methods by providing the following: (i) services for the preparation of genomic libraries and quantification of gene expression; (ii) high-performance liquid chromatography and mass spectrometry instrumentation for the analysis of lipids and metabolites; and (iii) facilities for the cultivation, isolation, and long-term storage of microbial strains. To expand Core capabilities and user base while continuing to support current and past COBRE researchers, MGAL services will be expanded to include metagenomic and transcriptomic library preparation, metabolomic analysis, and enhanced microbial biobanking facilities that will include the creation of a community searchable database containing information about endemic Hawaiian strains. The MGAL Core is available to researchers throughout the state of Hawai'i and visiting scientists, thus encouraging greater interaction within the local microbiome research community and seeding collaborative efforts from abroad. The facility will beneficially impact COBRE investigators and affiliates by providing essential infrastructure and expertise to advance research and foster the careers of junior faculty. Moreover, the Core will support the continued growth of UHM as a major center for microbiome studies in the Pacific Region by providing hands-on training in library preparation and metabolite analysis, hosting summer workshops on fundamental microbiome research methods, integrating with undergraduate and graduate laboratory classes, and facilitating outreach efforts to characterize Hawai'i’s microbiome.