Flow Cytometry

Project Summary/Abstract - FLOW CYTOMETRY SHARED RESOURCE The UNMCCC Flow Cytometry Shared Resource (Flow Cytometry) supports basic and translational cancer research by providing cost-effective, full service flow cytometry services and high-speed sterile sorting of cells, including the analysis of patient samples, to UNMCCC members and non-member users. Faculty Director Jennifer Gillette, Ph.D. (CMO) supervises the Resource, which is operated by a full-time core technical director, Wade Johnson, Ph.D. The UNMCCC Research Administration manages the Resource, which provides several types of flow cytometry services including instrumentation for complex multi-panel analysis as well as single cell sorting into microplate wells for cloning, PCR, and RNA-sequencing analysis. Reservations, billing and usage are tracked through a centralized on-line system. Additionally, the Resource manages the flow cytometry data analysis contract for the institution, which individual labs can buy into at a reduced price. The Resource Director and staff work closely with UNMCCC research programs to develop new methodologies that promote innovative basic and translational research goals. Moreover, the Resource actively promotes and disseminates information about new flow cytometry and data analysis techniques by maintaining an up-to-date web page and by giving presentations at UNMCCC meetings and retreats. Finally, the Resource Director and staff actively engage in the education mission of the UNMCCC through training and participation in several graduate level courses. During the previous 5-year project period, 58 UNMCCC members from 3 UNMCCC Research Programs were responsible for 75% of total Resource usage. Additionally, the Resource supported 146 publications and 128 peer-reviewed grants.