Investigating the Mechanical Properties of Vertebrate Notochord in Zebrafish

ABSTRACT The notochord is a conserved axial structure in all chordates that provides crucial structural support for the embryonic axis and, in vertebrates, the vertebral column or spine. Despite its significant role during development, the mechanical properties of the vertebrate notochord remain poorly understood. Unlike basal chordates, in which the notochord is a continuous pressurized tube, in vertebrates, the notochord's interior is made of large vacuolated cells. Each vacuolated cell contains one fluid-filled vacuole that occupies most of the cell volume. Vacuolated cells have been proposed to facilitate symmetrical vertebrae formation by absorbing the force of constricting vertebral bone growth on the notochord. Previous work showed that localized vacuolated cell defects lead to spine deformities at the location of the compromised cells, suggesting that the notochord can respond locally. Using acute mechanical stresses and genetic manipulations, I will investigate the structure's response to perturbations and the specific role of the vacuoles in this response. This research is complemented with a training plan that indlcudes didactic biology training and rigorous professional development within an interdisciplinary environment to enhance the breadth of knowledge and expertise of the trainee.