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NIHNATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCESR35

Mechanisms of mitochondrial mutation rate variation across eukaryotes

Active
Institution
Colorado State University, CO
Principal investigator
Daniel Benjamin Sloan
Funding
$40K
Duration
2023–2028
Open portfolio

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Abstract

PROJECT SUMMARY This administrative supplement will be used to replace broken equipment (an Agilent TapeStation) that is essential to the project funded by the parent award on mechanisms of mitochondrial mutation rate variation. The project relies on generation of specialized high-throughput DNA sequencing libraries with ultra-high fidelity to detect de novo mutations. These libraries must go through a quality control process, which includes analysis of size distribution and quantity with a TapeStation. The requested TapeStation 4150 unit will replace a TapeStation 2200 unit that is no longer operational and cannot be repaired because the manufacturer no longer supports the outdated model. The replacement will be used to complete project goals over the 3+ years remaining on the parent award.

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R35NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCESMaximizing Investigators' Research Award A Study Section[MRAA]longerlibrariesmutationparentreplacesize distributionunitmitochondrial

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