KOMP2-Phase3 Production and Phenotyping by the DTCC Consortium TCP Transfer to UC Davis

Transfer of KOMP2 Mice and Materials from The Center for Phenogenomics to UC Davis ABSTRACT This application from the Knockout Mouse Phenotyping Project (KOMP2) at the University of California, Davis proposes that UC Davis import mice for 26 lines that have not yet completed production and phenotyping at TCP. This is being proposed because TCP can no longer participate as a subcontractor on the KOMP2 project after the start of the next grant period (August 1) in order to be in compliance with the updated NIH Policy on Foreign Subawards (NOT-OD-25-104) issued May 1, 2026. These 26 mouse lines are novel and unique, and do not represent any mouse lines that UC Davis has already produced and/or phenotyped. Male and female mice that have been confirmed for germline transmission of the targeted allele at TCP will be packed and transferred in 4-5 shipments to UC Davis. Upon arrival, mice will undergo standard importation processing and confirmation of viability and identity, entered into the KOMP2 project database, raised and bred to produce adult male and female cohorts in the KOMP2 vivarium space, deposited into the MMRRC at UC Davis as cryopreserved sperm, and ultimately enrolled in the KOMP2 phenotyping pipeline at UC Davis. This approach will incur costs for shipping, processing, breeding, genotyping, and cryopreservation. The cryopreserved sperm will be integrated into the existing MMRRC inventory, making these mouse lines available for distribution to the biomedical research community, especially NIH funded investigators. The costs to execute this project are beyond the available support provided by the parent KOMP2 grant to UC Davis. If awarded, funds will be used to complete this project during the current grant year.