Biological Imaging Module

Biological Imaging (BI) Module Summary The Biological Imaging (BI) Module provides state-of-the-art equipment and resources for histological analysis and analytical imaging of biological tissues, for training vision research investigators in the use of these technologies, and to promote new collaborative studies among established and emerging investigators. Specific Aims are: 1) To provide state-of-the-art super-resolution technology for analytical imaging of ocular and neural tissues, which are beyond reach of individual laboratories. 2) To provide training for new investigators and staff on advanced imaging techniques in super-resolution semi-automated confocal microscopy, deconvolution and 3D imaging software and other module equipment and digital graphics production. 3) To promote research of early stage investigators and foster interactions and collaborations among a university-wide, multi-cultural, diverse vision research community. The BI module was extensively used in the last grant cycle and was essential for promoting innovative vision research, including testing efficacy of novel therapies, developing and validating viral expression vectors for gene therapy projects, investigating receptor-ligand interactions during disease, identifying biomarkers and validating proteomics/metabolomics data, as described below. BI module equipment was expanded in the last year to include new confocal microscopy systems, live retinal imaging and sensitive protein analysis. The BI module has been used to generate data that was reported in numerous publications and included in new grant awards and R01 applications from established and recently hired faculty. The module also facilitated recruitment of a new faculty member (Dr. Pham) who will be a heavy user of the confocal live imaging system. This module will continue to provide an essential resource for translational and basic science research for investigators of the McKnight Vision Research center (MVRC).